AKTIVITAS PROTEASE EKSTRASELULER BAKTERI YANG DIISOLASI DARI LIMBAH CAIR INDUSTRI TAHU

Tofu waste that is discharged into the aquatic environment has a negative impact on quality of water around tofu liquid disposal site. Industrial enzyme production currently dominated by proteases. Proteases in pharmaceutical field support absorption proteins digestive tract, as active cosmetic ingredients. This study aims to isolate, identify protease-producing bacteria from wastewater, determine total protein content and activity index 5 isolates bacteria. wastewater were isolated with Skim Milk Agar medium. bacterial selected which produced widest clear zones, different colony characters, identified morphological tests, gram staining biochemical tests. Five made suspension crude protease extract level was determined using Bicinchoninic Acid method, tested. colonies showed 1 2 included Gram positive bacteria, 3, 4 The value industrial five respectively: 219.93; 833,267; 421,267; 81,267; 38.6 µg/µL. highest proteolytic test sample 2.13.